ORGLIST: Basic digestion of RNA

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From: Feng Gu (fgu$##$bcmp.med.harvard.edu)
Date: Tue Jun 30 1998 - 23:23:56 EDT


Does anybody know the best conditions for digesting RNA oliogomer with
aqueous base (basic buffer? pH? etc). Because there is an ester on the
RNA (an adduct), the solution can't be too basic (e.g, pH > 13).
Thank's in advance.
Feng


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